2025
Norovirus co-opts NINJ1 for selective protein secretion
Song J, Zhang L, Moon S, Fang A, Wang G, Gheshm N, Loeb S, Cao P, Wallace J, Alfajaro M, Strine M, Beatty W, Jamieson A, Orchard R, Robinson B, Nice T, Wilen C, Orvedahl A, Reese T, Lee S. Norovirus co-opts NINJ1 for selective protein secretion. Science Advances 2025, 11: eadu7985. PMID: 40020060, PMCID: PMC11870086, DOI: 10.1126/sciadv.adu7985.Peer-Reviewed Original ResearchConceptsPlasma membrane ruptureDamage-associated molecular patternsNS1 secretionNinjurin-1Programmed cell deathAmino acid residuesViral replication sitesViral protein NS1CRISPR screensIntracellular viral proteinsMutagenesis studiesMembrane ruptureProtein NS1Unconventional pathwayCaspase-3Protein secretionViral proteinsReplication sitesCell deathMolecular patternsGenetic ablationNS1Pharmaceutical inhibitionDAMP releaseProtein
2024
Leishmania major surface components and DKK1 signalling via LRP6 promote migration and longevity of neutrophils in the infection site
Ihedioha O, Marcarian H, Sivakoses A, Beverley S, McMahon-Pratt D, Bothwell A. Leishmania major surface components and DKK1 signalling via LRP6 promote migration and longevity of neutrophils in the infection site. Frontiers In Immunology 2024, 15: 1473133. PMID: 39502693, PMCID: PMC11534728, DOI: 10.3389/fimmu.2024.1473133.Peer-Reviewed Original ResearchParasite-infected miceBALB/c miceDickkopf-1Infection siteInfected BALB/c micePathogen-associated molecular patternsWild-type BALB/c miceLevels of apoptosisNull mutantsLeishmania infectionDickkopf-1 levelsDraining lymph nodesNeutrophil-platelet aggregatesLeukocyte-platelet aggregatesInfiltration of neutrophilsReduced neutrophil activationDay 3 PIResponse to infectionSignaling pathwayHost-related factorsMolecular patternsMembrane componentsLRP6Lymph nodesTh2 differentiationProgrammable Bacteria‐Based Biohybrids as Living Biotherapeutics for Enhanced Cancer Sonodynamic‐Immunotherapy
Wang C, Chen L, Zhu J, Wang C, Li M, Miao Y, Liu N, Ji Z, Pan F, Liu Y, Zhu J, Yang Y, Chen Q. Programmable Bacteria‐Based Biohybrids as Living Biotherapeutics for Enhanced Cancer Sonodynamic‐Immunotherapy. Advanced Functional Materials 2024, 34 DOI: 10.1002/adfm.202316092.Peer-Reviewed Original ResearchSonodynamic therapyAntitumor immune responseTumor-associated antigensImmune memory responsePrevent tumor recurrenceInhibit tumor metastasisOrthotopic colorectal tumorsAbscopal effectTumor recurrenceEffective sonodynamic therapyTumor siteNano-sonosensitizersTumor hypoxiaPathogen-associated molecular patternsColorectal tumorsMemory responsesTherapeutic delivery systemsTumor metastasisImmune responseTumorLive biotherapeuticsMetastasisBacterial pathogen-associated molecular patternsMolecular patternsOverexpressing catalaseInternational consensus guidelines for the definition, detection, and interpretation of autophagy-dependent ferroptosis
Chen X, Tsvetkov A, Shen H, Isidoro C, Ktistakis N, Linkermann A, Koopman W, Simon H, Galluzzi L, Luo S, Xu D, Gu W, Peulen O, Cai Q, Rubinsztein D, Chi J, Zhang D, Li C, Toyokuni S, Liu J, Roh J, Dai E, Juhasz G, Liu W, Zhang J, Yang M, Liu J, Zhu L, Zou W, Piacentini M, Ding W, Yue Z, Xie Y, Petersen M, Gewirtz D, Mandell M, Chu C, Sinha D, Eftekharpour E, Zhivotovsky B, Besteiro S, Gabrilovich D, Kim D, Kagan V, Bayir H, Chen G, Ayton S, Lünemann J, Komatsu M, Krautwald S, Loos B, Baehrecke E, Wang J, Lane J, Sadoshima J, Yang W, Gao M, Münz C, Thumm M, Kampmann M, Yu D, Lipinski M, Jones J, Jiang X, Zeh H, Kang R, Klionsky D, Kroemer G, Tang D. International consensus guidelines for the definition, detection, and interpretation of autophagy-dependent ferroptosis. Autophagy 2024, 20: 1213-1246. PMID: 38442890, PMCID: PMC11210914, DOI: 10.1080/15548627.2024.2319901.Peer-Reviewed Original ResearchAutophagy-dependent ferroptosisCell deathElectronparamagnetic resonanceTransmission electron microscopyEnergy transferDanger/damage-associated molecular patternsOxidized formComplex degradation processFerroptotic cell deathElectron microscopyDegradation processEndoplasmic reticulumResponse elementAntioxidant response elementReactive oxygen speciesEpithelial-mesenchymal transitionMolecular patternsCellular defenseCell typesPhenAutophagyOxygen speciesPolyunsaturated fatty acidsEPRFerroptotic damage
2023
TMIC-20. A SPATIALLY RESOLVED HUMAN GLIOBLASTOMA ATLAS REVEALS DISTINCT CELLULAR AND MOLECULAR PATTERNS OF ANATOMICAL NICHES
Shah N, Park H, Sonpatki P, Han K, Yu H, Kim S, Chowdhury T, Byun Y, Kang H, Lee J, Lee S, Won J, Kim T, Choi S, Shin Y, Ku J, Lee S, Yun H, Park S, Park C, Park W. TMIC-20. A SPATIALLY RESOLVED HUMAN GLIOBLASTOMA ATLAS REVEALS DISTINCT CELLULAR AND MOLECULAR PATTERNS OF ANATOMICAL NICHES. Neuro-Oncology 2023, 25: v282-v282. PMCID: PMC10639919, DOI: 10.1093/neuonc/noad179.1086.Peer-Reviewed Original ResearchCellular componentsBulk RNA-seq dataAnatomical nichesSingle-cell atlasSingle-cell RNARNA-seq dataMulti-omics profilingDifferent cellular componentsInteraction networksSpatial transcriptomeCellular heterogeneitySpatial interaction networkDistinct cellularMolecular patternsCellular architectureNicheUnrecognized subtypesSpatial organizationAbstract GlioblastomaValuable resourceGlioma samplesStromal cellsEffective combinatorial therapiesComprehensive insightGlioblastomaThe VCAM1–ApoE pathway directs microglial chemotaxis and alleviates Alzheimer’s disease pathology
Lau S, Wu W, Wong H, Ouyang L, Qiao Y, Xu J, Lau J, Wong C, Jiang Y, Holtzman D, Fu A, Ip N. The VCAM1–ApoE pathway directs microglial chemotaxis and alleviates Alzheimer’s disease pathology. Nature Aging 2023, 3: 1219-1236. PMID: 37735240, PMCID: PMC10570140, DOI: 10.1038/s43587-023-00491-1.Peer-Reviewed Original ResearchConceptsDanger-associated molecular patternsAlzheimer's diseaseMicroglial chemotaxisAmeliorate AD pathologyDisease pathologyAlzheimer's disease pathologyAmyloid-betaFunctional screeningPlaque-associatedMicroglial clearanceAD pathologyExtrinsic signalsPhagocytic clearanceExacerbate disease pathologyMolecular patternsIL-33ChemotaxisMicroglial functionAlzheimerVCAM1Interleukin-33Higher cerebrospinal fluid levelsApoPathwayMicrogliaReceptor–ligand interaction controls microglial chemotaxis and amelioration of Alzheimer's disease pathology
Lau S, Fu A, Ip N. Receptor–ligand interaction controls microglial chemotaxis and amelioration of Alzheimer's disease pathology. Journal Of Neurochemistry 2023, 166: 891-903. PMID: 37603311, DOI: 10.1111/jnc.15933.Peer-Reviewed Original ResearchConceptsDanger-associated molecular patternsMicroglial chemotaxisAD pathogenesisAlzheimer's diseaseMicroglial functionRepertoire of surface receptorsHyperphosphorylated tauAlzheimer's disease pathologyAmyloid-betaMolecular machineryMicroglial receptorsReceptor-ligand interactionsPhagocytic clearanceReceptor-ligand axisMolecular patternsSurface receptorsFunctional transitionDisease pathologyChemotaxisBrain homeostasisAberrant synaptic pruningClearance activityCritical stepsReceptorsAmyloid
2022
Placental macrophage responses to viral and bacterial ligands and the influence of fetal sex
Pantazi P, Kaforou M, Tang Z, Abrahams V, McArdle A, Guller S, Holder B. Placental macrophage responses to viral and bacterial ligands and the influence of fetal sex. IScience 2022, 25: 105653. PMID: 36505933, PMCID: PMC9732417, DOI: 10.1016/j.isci.2022.105653.Peer-Reviewed Original ResearchPathogen-associated molecular patternsViral pathogen-associated molecular patternsHofbauer cellsFetal sexMajor histocompatibility complex class IAdverse pregnancy outcomesHistocompatibility complex class IToll-like receptorsComplex class ISex-dependent responseInfectious triggerPregnancy outcomesPlacental macrophagesVertical pathogen transmissionViral infectionLipid metabolismMacrophage responseBacterial ligandsProtein expression changesClass IMolecular patternsPlacentaMacrophagesCytoskeleton organizationQuantitative proteomicsMucus sialylation determines intestinal host-commensal homeostasis
Yao Y, Kim G, Shafer S, Chen Z, Kubo S, Ji Y, Luo J, Yang W, Perner SP, Kanellopoulou C, Park AY, Jiang P, Li J, Baris S, Aydiner EK, Ertem D, Mulder DJ, Warner N, Griffiths AM, Topf-Olivestone C, Kori M, Werner L, Ouahed J, Field M, Liu C, Schwarz B, Bosio CM, Ganesan S, Song J, Urlaub H, Oellerich T, Malaker SA, Zheng L, Bertozzi CR, Zhang Y, Matthews H, Montgomery W, Shih HY, Jiang J, Jones M, Baras A, Shuldiner A, Gonzaga-Jauregui C, Snapper SB, Muise AM, Shouval DS, Ozen A, Pan KT, Wu C, Lenardo MJ. Mucus sialylation determines intestinal host-commensal homeostasis. Cell 2022, 185: 1172-1188.e28. PMID: 35303419, PMCID: PMC9088855, DOI: 10.1016/j.cell.2022.02.013.Peer-Reviewed Original ResearchConceptsInflammatory bowel diseaseMicrobial pathogen-associated molecular patternsPathogen-associated molecular patternsMicrobial symbiosisRegulatory networksIntestinal mucusGlycoproteomic profilingMolecular patternsTerminal sialylationMucus proteinsProteolytic degradationBiochemical analysisBacterial invasionBowel diseaseIntestinal inflammationMucus integrityMutationsSialylationST6GALNAC1Barrier integrityGoblet cellsMucus barrierFirst lineSymbiosisCommensalismAdvanced Glycation End Products-Mediated Oxidative Stress and Regulated Cell Death Signaling in Cancer
Pathak C, Vaidya F, Waghela B, Chhipa A, Tiwari B, Ranjan K. Advanced Glycation End Products-Mediated Oxidative Stress and Regulated Cell Death Signaling in Cancer. 2022, 535-550. DOI: 10.1007/978-981-15-9411-3_44.ChaptersRegulated cell deathCell deathDanger-associated molecular patternsOxidative stressTranscription factorsMolecular mechanismsCancer progressionCellular dysfunctionMolecular patternsRedox imbalanceCancer cellsPathological consequencesTypes of cancerROS generationCurrent understandingAGEs/RAGENucleic acidsAdvanced glycation end productsEnd productsGlycation of proteinsRAGE interactionAccumulationPathophysiological effectsGenesGlycation end products
2021
Evolving Perspectives on Innate Immune Mechanisms of IPF
Ishikawa G, Liu A, Herzog EL. Evolving Perspectives on Innate Immune Mechanisms of IPF. Frontiers In Molecular Biosciences 2021, 8: 676569. PMID: 34434962, PMCID: PMC8381017, DOI: 10.3389/fmolb.2021.676569.Peer-Reviewed Original ResearchIdiopathic pulmonary fibrosisInnate immunityInnate immune populationsMolecular patternsMyeloid suppressor cellsInnate lymphoid cellsInnate immune mechanismsEpithelial-fibroblast interactionsRole of substancesSuppressor cellsPulmonary fibrosisImmune populationsImmune mechanismsDisease outcomePotential therapyLymphoid cellsHuman studiesFibrotic microenvironmentCommensal microbesAnimal modelingGenetic factorsImmunityFuture studiesComplex roleCellsCytokine signaling convergence regulates the microglial state transition in Alzheimer’s disease
Lau S, Fu A, Ip N. Cytokine signaling convergence regulates the microglial state transition in Alzheimer’s disease. Cellular And Molecular Life Sciences 2021, 78: 4703-4712. PMID: 33847763, PMCID: PMC8195901, DOI: 10.1007/s00018-021-03810-0.Peer-Reviewed Original ResearchConceptsMicroglial state transitionCytokine signaling pathwaysDanger-associated molecular patternsAlzheimer's diseaseSignaling pathwayPathogenesis of Alzheimer's diseaseAD therapeutic developmentTherapeutic developmentHyperphosphorylated tauBeta-amyloidGenetic analysisAD pathologyCytokine signalingMolecular basisAD progressionMolecular patternsMicroglial dysfunctionSignal convergenceMicroglial responseMicroglial functionStimulated microgliaFunctional stateActive statePathwayChronic activationChapter 12 Innate immunity in SLE pathogenesis
Kang I. Chapter 12 Innate immunity in SLE pathogenesis. 2021, 181-188. DOI: 10.1016/b978-0-12-820583-9.00025-7.Peer-Reviewed Original ResearchDanger-associated molecular patternsSystemic lupus erythematosusInnate immune cellsToll-like receptorsPattern recognition receptorsDendritic cellsSelf-nucleic acidsImmune cellsGermline-encoded pattern recognition receptorsMultiple inflammatory pathwaysMolecular patternsNuclear factor kappa BAntigen-presenting cellsArray of cytokinesFactor kappa BPathogen-associated molecular patternsCell-intrinsic alterationsLupus erythematosusSLE pathogenesisInflammatory pathwaysInflammatory eventsInterferon regulatory factorIntrinsic alterationsKappa BInnate immunity
2020
Detection of Immunogenic Cell Death in Tumor Vaccination Mouse Model
Tatsuno K, Han P, Edelson R, Hanlon D. Detection of Immunogenic Cell Death in Tumor Vaccination Mouse Model. Methods In Molecular Biology 2020, 2255: 171-186. PMID: 34033103, DOI: 10.1007/978-1-0716-1162-3_15.Peer-Reviewed Original ResearchConceptsImmunogenic cell deathTumor cellsImmune responseHigh mobility box 1Antitumor immune responseCell deathType I IFNVaccination assayTLR agonistsVivo vaccinationICD inducersICD inductionMouse modelI IFNBox 1Animal modelsGold standard methodPreparation of vaccinesMolecular patternsRegulated cell deathHallmark featureDeathIntracellular chaperoneCellsSurface translocationMitochondrial antiviral signaling protein is crucial for the development of pulmonary fibrosis
Kim SH, Lee JY, Yoon CM, Shin HJ, Lee SW, Rosas I, Herzog E, Dela Cruz C, Kaminski N, Kang MJ. Mitochondrial antiviral signaling protein is crucial for the development of pulmonary fibrosis. European Respiratory Journal 2020, 57: 2000652. PMID: 33093124, PMCID: PMC8559259, DOI: 10.1183/13993003.00652-2020.Peer-Reviewed Original ResearchConceptsDamage-associated molecular patternsIdiopathic pulmonary fibrosisPulmonary fibrosisMAVS aggregationMultiple damage-associated molecular patternsExperimental pulmonary fibrosisMitochondrial antiviral signaling proteinInnate immune responseIPF patientsMAVS signalingIPF treatmentBleomycin injuryLung fibrosisTherapeutic effectImmune responseTherapeutic strategiesMAVS expressionFibrosisDanger signalsCritical mediatorMolecular patternsABT-263LungInjuryBH3 mimeticsChapter 3 Infection and host response
Fink S, Campbell S. Chapter 3 Infection and host response. 2020, 33-53. DOI: 10.1016/b978-0-12-813257-9.00003-6.Peer-Reviewed Original ResearchMammalian immune systemModel organismsMolecular conversationMammalian immunityElegant mechanismAstonishing arrayMolecular elementsPathogenic microbesImmune systemMolecular patternsMicrobesInnate immunityPathogensComplex mechanismsAdaptive immunityEffector mechanismsHostHost responseHuman infectionsCell damageImmunityInfectionTarget-specific recognitionOrganismsMechanism
2019
Innate Immunity: Recognition and Effector Functions
Kavathas P, Krause P, Ruddle N. Innate Immunity: Recognition and Effector Functions. 2019, 39-53. DOI: 10.1007/978-3-030-25553-4_3.ChaptersDanger-associated molecular patternsPathogen-associated molecular patternsTypes of pathogensMolecular patternsInducer of inflammationSecretion of cytokinesTypes of receptorsChronic inflammationInflammatory responseEffector mechanismsEffector functionsInnate immunityInflammationComplement systemComplement proteinsMicrobial invasionReceptorsFighting infectionsInfectionCell-cell communicationSecretionCell stressNucleic acidsPathogensCellsA cell-penetrating peptide blocks Toll-like receptor-mediated downstream signaling and ameliorates autoimmune and inflammatory diseases in mice
Kwon HK, Patra MC, Shin HJ, Gui X, Achek A, Panneerselvam S, Kim DJ, Song SJ, Hong R, Kim KS, Kim YG, Lee FY, Hahm DH, Lee SH, Choi S. A cell-penetrating peptide blocks Toll-like receptor-mediated downstream signaling and ameliorates autoimmune and inflammatory diseases in mice. Experimental & Molecular Medicine 2019, 51: 1-19. PMID: 31028244, PMCID: PMC6486608, DOI: 10.1038/s12276-019-0244-0.Peer-Reviewed Original ResearchConceptsToll-like receptorsCollagen-induced arthritisInflammatory diseasesRodent modelsOverexpression of TLRsMultiple Toll-like receptorsAutoimmune/inflammatory diseasesSecretion of lipopolysaccharideDamage-associated molecular patternsInflammatory signaling cascadesIL-1β secretionNumerous inflammatory diseasesRheumatoid arthritisProinflammatory cytokinesLiver damageTLR4 TIR domainImmune responseAnimal studiesTherapeutic potentialToll/interleukinMolecular patternsMRNA levelsDiseaseArthritisSelective inhibitor
2018
An Antiviral Branch of the IL-1 Signaling Pathway Restricts Immune-Evasive Virus Replication
Orzalli MH, Smith A, Jurado KA, Iwasaki A, Garlick JA, Kagan JC. An Antiviral Branch of the IL-1 Signaling Pathway Restricts Immune-Evasive Virus Replication. Molecular Cell 2018, 71: 825-840.e6. PMID: 30100266, PMCID: PMC6411291, DOI: 10.1016/j.molcel.2018.07.009.Peer-Reviewed Original ResearchConceptsDamage-associated molecular patternsIL-1Host-derived damage-associated molecular patternsViral replicationVirus replicationInfected cellsInterleukin-1 family cytokinesIL-1 Signaling PathwayInflammatory gene expressionIL-1 actsHuman skin explantsProtective immunityIL-1αBarrier defenseInflammatory signalsViral infectionFamily cytokinesSkin explantsGene expressionMolecular patternsSkin fibroblastsSignaling pathwaysAntiviral systemBarrier epitheliaCell typesThe Role of Immune and Inflammatory Cells in Idiopathic Pulmonary Fibrosis
Desai O, Winkler J, Minasyan M, Herzog EL. The Role of Immune and Inflammatory Cells in Idiopathic Pulmonary Fibrosis. Frontiers In Medicine 2018, 5: 43. PMID: 29616220, PMCID: PMC5869935, DOI: 10.3389/fmed.2018.00043.BooksIdiopathic pulmonary fibrosisPulmonary fibrosisDanger-associated molecular patternsInnate immune populationsMolecular patternsMyeloid suppressor cellsInnate lymphoid cellsProfessional immune cellsT helper cellsAdaptive immune responsesRole of ImmuneAdaptive immune processesPathogen-associated molecular patternsImmunopathogenic mechanismsSuppressor cellsImmune activationInflammatory cellsImmune populationsImmune cellsDisease outcomeImmune responseFibrotic responsePotential therapyLymphoid cellsHuman studies
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