2025
Salidroside Improves Oocyte Competence of Reproductively Old Mice by Enhancing Mitophagy
Gu J, Hua R, Wu H, Guo C, Hai Z, Xiao Y, Yeung W, Liu K, Babayev E, Wang T. Salidroside Improves Oocyte Competence of Reproductively Old Mice by Enhancing Mitophagy. Aging Cell 2025, 24: e14475. PMID: 39789811, PMCID: PMC12073897, DOI: 10.1111/acel.14475.Peer-Reviewed Original ResearchReproductively old miceImprove oocyte qualityOocyte qualityOld miceDecline of oocyte qualityDevelopmental competence of oocytesReproductively older womenCompetence of oocytesKnowledge of therapeutic optionsOocyte competenceDevelopmental competenceTherapeutic optionsMaternal ageSpindle/chromosome structureAssisted reproductionOld femaleMiceOocytesOlder womenNatural fertilityFemale fertilityMitochondrial function
2024
Looking into the future: a machine learning powered prediction model for oocyte return rates after cryopreservation
Fouks Y, Bortoletto P, Chang J, Penzias A, Vaughan D, Sakkas D. Looking into the future: a machine learning powered prediction model for oocyte return rates after cryopreservation. Reproductive BioMedicine Online 2024, 50: 104432. PMID: 39642702, DOI: 10.1016/j.rbmo.2024.104432.Peer-Reviewed Original ResearchSociety for Assisted Reproductive TechnologyDiminished ovarian reserveStored oocytesUS fertility clinicsAge-related infertilityAssisted reproductive technologyFertility clinicsLikelihood of patientsOocyte cryopreservationUnexplained infertilityInfertility diagnosisOvarian reservePatient agePatient demographicsTreatment indicationsPatient counselingReproductive technologyFertility diagnosisPatientsOocytesGender-relatedInfertilitySelf-financingClinicDiagnosisMaximizing Donor Egg Efficiency: Artificial Intelligence and Genetically Certified Oocytes
Aydin B, Hudkova D, Halicigil C. Maximizing Donor Egg Efficiency: Artificial Intelligence and Genetically Certified Oocytes. 2024, 471-490. DOI: 10.1007/978-3-031-58214-1_48.Peer-Reviewed Original ResearchDonor oocyte banksSelection of oocytesOocyte bankingOocyte vitrificationOocyte donationPGT-A methodsChances of implantationVitrification protocolPolar body biopsyUsable blastocystsEuploid blastocystsPGT-AAneuploid oocytesClinical pregnancyBody biopsyAneuploidy statusBiopsy methodClinical dataOncological problemSurvival rateOocytesPolar bodyPatientsMorphological evaluationMedical reasonsThe role of declining ataxia-telangiectasia-mutated (ATM) function in oocyte aging
Suzuki R, Tan X, Szymanska K, Kubikova N, Perez C, Wells D, Oktay K. The role of declining ataxia-telangiectasia-mutated (ATM) function in oocyte aging. Cell Death Discovery 2024, 10: 302. PMID: 38914566, PMCID: PMC11196715, DOI: 10.1038/s41420-024-02041-z.Peer-Reviewed Original ResearchOocyte qualityAtaxia telangiectasia-mutatedOocyte agingMaintenance of oocyte qualityDNA double-strand breaksMeiosis in vitroPoor embryo developmentImpaired oocyte qualityATM functionMolecular cytogenetic analysisFormation of DNA DSBsDeath compared to controlsBlastocyst stageEmbryo cleavageDNA DSBsOvarian agingCytogenetic analysisResistance to genotoxic stressActive metaboliteNext-generation sequencingRates compared to controlsIn vitro cultureOocytesDNA DSB repairAge-induced declineDecision-making in women who considered planned oocyte cryopreservation: decision satisfaction or regret?
Korkidakis A, Martinez S, Sabbagh R, Heyward Q, Sakkas D, Domar A, Toth T. Decision-making in women who considered planned oocyte cryopreservation: decision satisfaction or regret? Reproductive BioMedicine Online 2024, 50: 103944. PMID: 39799100, DOI: 10.1016/j.rbmo.2024.103944.Peer-Reviewed Original ResearchPlanned OCPlanned oocyte cryopreservationOocyte cryopreservationDecision Regret ScaleDecision Regret Scale scoresDecision regretCryopreserved oocytesDonor spermREDCap platformMultivariate modelEligibility criteriaReproductive plansProportion of respondentsDRS scoresDecreased numberWomenRegret ScaleOocytesCryopreservationScoresGroupMitochondrial Unfolded Protein Response Gene Clpp Is Required for Oocyte Function and Female Fertility
Ergun Y, Imamoglu A, Cozzolino M, Demirkiran C, Basar M, Garg A, Yildirim R, Seli E. Mitochondrial Unfolded Protein Response Gene Clpp Is Required for Oocyte Function and Female Fertility. International Journal Of Molecular Sciences 2024, 25: 1866. PMID: 38339144, PMCID: PMC10855406, DOI: 10.3390/ijms25031866.Peer-Reviewed Original ResearchConceptsCaseinolytic peptidase PMouse modelProtein homeostasisStress responseUnfolded protein stress responseProtein stress responseCumulus/granulosa cellsOocyte competenceOocyte functionGlobal deletionFunctional abnormalitiesGenes clpPMetabolic stress responseFemale subfertilityFemale infertilityOocyte-specificOocytesReproductive functionMtUPRMiceProtein degradationReproductive competenceFemale fertilityDeletionHomeostasis
2023
The Aging Ovary and the Tales Learned Since Fetal Development
Lopez J, Hohensee G, Liang J, Sela M, Johnson J, Kallen A. The Aging Ovary and the Tales Learned Since Fetal Development. Sexual Development 2023, 17: 156-168. PMID: 37598664, PMCID: PMC10841896, DOI: 10.1159/000532072.Peer-Reviewed Original ResearchConceptsOvarian agingGerm cell populationsQuality of oocytesTreatment of ovarian agingStem cell exhaustionOocyte behaviorWoman's reproductive lifespanFollicle poolOvarian reserveFetal lifeFetal developmentAging ovariesReproductive ageCell exhaustionCell populationsOocytesFolliclesOvaryReproductive lifespanGenomic instabilityOverall agingHallmarks of agingAgeTelomere attritionDNA damageOocyte Cryopreservation for Medical and Planned Indications: A Practical Guide and Overview
Han E, Seifer D. Oocyte Cryopreservation for Medical and Planned Indications: A Practical Guide and Overview. Journal Of Clinical Medicine 2023, 12: 3542. PMID: 37240648, PMCID: PMC10218997, DOI: 10.3390/jcm12103542.Peer-Reviewed Original ResearchOocyte cryopreservationAge-related fertility declineOvarian follicular lossFirst successful pregnancyElective oocyte cryopreservationFollicular lossGonadotoxic therapySuccessful pregnancyTreatment of cancerBiologic childrenFollicular fluidOvarian folliclesNarrative reviewMature oocytesOocytesPregnancyPatientsTherapyCancerFolliclesIn Vitro Gametogenesis in Oncofertility: A Review of Its Potential Use and Present-Day Challenges in Moving toward Fertility Preservation and Restoration
Wesevich V, Arkfeld C, Seifer D. In Vitro Gametogenesis in Oncofertility: A Review of Its Potential Use and Present-Day Challenges in Moving toward Fertility Preservation and Restoration. Journal Of Clinical Medicine 2023, 12: 3305. PMID: 37176745, PMCID: PMC10179531, DOI: 10.3390/jcm12093305.Peer-Reviewed Original ResearchPluripotent stem cellsPrimordial germ cell-like cellsGerm cell-like cellsStem cell biology researchStem cellsCell biology researchCell-like cellsVitro gametogenesisBiology researchReproductive lifespanGametogenesisFibroblast cellsDermal fibroblast cellsCellsReprogrammingEmbryogenesisOogoniaEmbryosViable oocytesFertilityReproductionSpermatocytesCurrent fertility preservation optionsOocytesEx vivoMitochondrial stress response gene Clpp deficiency impairs oocyte competence and deteriorate cyclophosphamide-induced ovarian damage in young mice
Li G, Gu J, Zhou X, Wu T, Li X, Hua R, Hai Z, Xiao Y, Su J, Yeung W, Liu K, Guo C, Wang T. Mitochondrial stress response gene Clpp deficiency impairs oocyte competence and deteriorate cyclophosphamide-induced ovarian damage in young mice. Frontiers In Endocrinology 2023, 14: 1122012. PMID: 37033217, PMCID: PMC10081448, DOI: 10.3389/fendo.2023.1122012.Peer-Reviewed Original ResearchConceptsCyclophosphamide-induced ovarian damageOvarian damageOocyte-specific deletionOocyte competencePremature ovarian insufficiencyImpaired oocyte competenceChemotherapeutic drug cyclophosphamideCaseinolytic peptidase PDecreased mitochondrial membrane potentialAneuploidy rateMII oocytesCTX treatmentElevated ROS levelsOvarian insufficiencyEarly folliculogenesisKnockout miceYoung miceMitochondrial membrane potentialMouse modelDrug cyclophosphamideTreat cancerMiceOocytesSpindle rateMembrane potential
2022
Development of an Open Microfluidic Platform for Oocyte One-Stop Vitrification with Cryotop Method
Miao S, Guo C, Jiang Z, Wei H, Jiang X, Gu J, Hai Z, Wang T, Liu Y. Development of an Open Microfluidic Platform for Oocyte One-Stop Vitrification with Cryotop Method. Biosensors 2022, 12: 766. PMID: 36140151, PMCID: PMC9496857, DOI: 10.3390/bios12090766.Peer-Reviewed Original ResearchConceptsCryotop methodMitochondrial membrane potentialMicrofluidic systemReactive oxygen speciesLevels of oocytesEffect of vitrificationCryoprotective agentsOocyte vitrificationRisk of cell lossOpen microfluidic systemTreated oocytesFertility preservationCell transferCryotopReproductive centerCell lossSurvival rateOpen microfluidic chipOocytesAssisted reproductionSufficient cooling rateMembrane potentialProcessing chipOpen microfluidic platformAutomatic microfluidic systemTargeted Deletion of Mitofusin 1 and Mitofusin 2 Causes Female Infertility and Loss of Follicular Reserve
Cozzolino M, Ergun Y, Seli E. Targeted Deletion of Mitofusin 1 and Mitofusin 2 Causes Female Infertility and Loss of Follicular Reserve. Reproductive Sciences 2022, 30: 560-568. PMID: 35739352, DOI: 10.1007/s43032-022-01014-w.Peer-Reviewed Original ResearchConceptsMitofusin 1Mitofusin 2Double deletionFemale reproductive competencePotential functional redundancyDynamic organellesCellular homeostasisFunctional redundancyMitochondrial dynamicsEnvironmental stressMitochondrial functionMitochondrial dysfunctionMfn1Reproductive competenceTargeted deletionMfn2Oocyte maturationDeletionCritical roleReproductive agingFemale infertilityOocytesOocyte qualityFusion mechanismMitofusinsMitochondrial dysfunction caused by targeted deletion of Mfn1 does not result in telomere shortening in oocytes.
Cozzolino M, Seli E. Mitochondrial dysfunction caused by targeted deletion of Mfn1 does not result in telomere shortening in oocytes. Zygote 2022, 30: 735-737. PMID: 35730364, DOI: 10.1017/s0967199422000089.Peer-Reviewed Original ResearchConceptsMitochondrial dysfunctionMaintenance of telomeresTargeted deletionEnd-protection functionTTAGGG repeatsMitochondrial fusionTelomeric repeatsSomatic cellsMitofusin 1Reactive oxygen speciesEnzyme complexWild-type miceOocyte growthDNA damageMouse oocytesTelomerase activityOocyte maturationDeletionFollicular depletionOxygen speciesTelomere lengthTelomeresFollicular developmentOocytesRepeatsCytoskeletal form and function in mammalian oocytes and zygotes
Dunkley S, Scheffler K, Mogessie B. Cytoskeletal form and function in mammalian oocytes and zygotes. Current Opinion In Cell Biology 2022, 75: 102073. PMID: 35364486, DOI: 10.1016/j.ceb.2022.02.007.Peer-Reviewed Original ResearchConceptsCytoskeletal formActin-microtubule interactionsReproductive cell biologyMammalian oocytesActin polymersMicrotubule cytoskeletonChromosome segregationChromosome structureF-actinSubcellular locationCytoskeletal systemSpindle self-organizationMammalian embryo developmentMicroscopy assaysCell biologyEmbryo developmentZygotesOocytesChromosomeAge-related changesCytoskeletalActinBiology
2021
Oxidation sensitizes TRPV2 to chemical and heat stimuli, but not mechanical stimulation
Oda M, Fujiwara Y, Ishizaki Y, Shibasaki K. Oxidation sensitizes TRPV2 to chemical and heat stimuli, but not mechanical stimulation. Biochemistry And Biophysics Reports 2021, 28: 101173. PMID: 34841092, PMCID: PMC8605382, DOI: 10.1016/j.bbrep.2021.101173.Peer-Reviewed Original ResearchTransient receptor potential vanilloid 2Response to 2-APBTransient receptor potential vanilloid 2 activationOocyte expression systemTwo-electrode voltage clamp recordingsMechanical stimulationVoltage clamp recordingsChemical ligandsOxidant chloramine TNoxious heatClamp recordingsOxidation of methionine residuesHeat stimuliTRP channelsTwo-electrodeOxidationLigandExpression systemStimulationChloramine TOocytesCell expression systemMethionine residuesMammalian cell expression systemChemicalMicroinjection of Xenopus tropicalis Embryos.
Lane M, Mis EK, Khokha MK. Microinjection of Xenopus tropicalis Embryos. Cold Spring Harbor Protocols 2021, 2022: pdb.prot107644. PMID: 34244348, DOI: 10.1101/pdb.prot107644.Peer-Reviewed Original ResearchConceptsEmbryo sizeLarger embryo sizeTargeted gene manipulationEmbryo development timeSmall embryo sizeXenopus tropicalis embryosDevelopmental biologyEarly embryosMicroinjection protocolGene manipulationGenetic studiesFirst divisionXenopusEmbryosImportant modelDevelopment timeMicroinjectionMorpholinoCRISPRBiologySpeciesDNAMRNAOocytesFertilization
2020
Advances and surprises in a decade of oocyte meiosis research.
Mogessie B. Advances and surprises in a decade of oocyte meiosis research. Essays In Biochemistry 2020, 64: 263-275. PMID: 32538429, DOI: 10.1042/ebc20190068.Peer-Reviewed Original Research
2019
Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes
Mogessie B. Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes. Methods In Molecular Biology 2019, 2101: 267-295. PMID: 31879910, PMCID: PMC9703194, DOI: 10.1007/978-1-0716-0219-5_17.Peer-Reviewed Original ResearchConceptsSpindle actinChromosome segregationAccurate chromosome segregationImmunofluorescence microscopy assayMammalian oocytesActin filamentsSpindle machineryMicroscopy assaysActinOocyte meiosisFunctional analysisMeiotic spindleChromosomeSegregationEukaryotesSpindleOocytesMeiosisMicrotubulesMachineryFilamentsAssayA step towards the automation of intracytoplasmic sperm injection: real time confirmation of mouse and human oocyte penetration and viability by electrical resistance measurement
Mor A, Zhang M, Esencan E, Simsek B, Nichols-Burns SM, Liu Y, Lo J, Kelk DA, Flores V, Gao XB, Seli E. A step towards the automation of intracytoplasmic sperm injection: real time confirmation of mouse and human oocyte penetration and viability by electrical resistance measurement. Fertility And Sterility 2019, 113: 234-236. PMID: 31883732, DOI: 10.1016/j.fertnstert.2019.09.023.Peer-Reviewed Original ResearchConceptsIntracytoplasmic sperm injectionICSI pipetteSperm injectionOocyte penetrationIntact oolemmaOocyte retrieval procedureMann-Whitney testMouse oocytesOocyte retrievalFemale miceMAIN OUTCOMEAcademic centersImmature oocytesHuman oocytesMature oocytesObjective toolMiceOocytesWomenVisual assessmentPipetteInjectionOolemmaStep 1GroupCl− and H+ coupling properties and subcellular localizations of wildtype and disease-associated variants of the voltage-gated Cl−/H+ exchanger ClC-5
Chang M, Brown M, Liu Y, Gainullin V, Harris P, Romero M, Lieske J. Cl− and H+ coupling properties and subcellular localizations of wildtype and disease-associated variants of the voltage-gated Cl−/H+ exchanger ClC-5. Journal Of Biological Chemistry 2019, 295: 1464-1473. PMID: 31852738, PMCID: PMC7008381, DOI: 10.1074/jbc.ra119.011366.Peer-Reviewed Original ResearchConceptsWT ClC-5Dent disease 1ClC-5ClC-5 transportEndosomal acidificationVoltage-gating propertiesIon-selective microelectrodesNonsense mutationDisease 1Disease-associated variantsExchange ratioSubcellular distributionEarly endosomesMutationsCo-localizationEndoplasmic reticulumVariantsTraffickingOocytesEndosomesSubcellular localization
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